SV40大腫瘍抗原は,いくつかの細胞のトランスクリプトを肯定的な方法で調節することができます
Cell
|September 1, 1982
まとめ
シミアンウイルス40 (SV40) 大型T抗原は,細胞伝達 RNA (mRNA) レベルを調節する. この研究は,特に許容的な条件下で,SV40変異細胞で上調される特定の細胞mRNAを特定しました.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 細胞生物学 細胞生物学
背景:
- SV40変換は細胞の遺伝子発現を変化させます.
- ウイルスの大型T抗原は,変容の重要な原動力である.
- ウイルスタンパク質による宿主遺伝子の調節を理解することは極めて重要です.
研究 の 目的:
- SV40変換によって調節される細胞のmRNAを特定するために.
- ホストの遺伝子発現を調節するSV40大T抗原の役割を調査する.
- SV40tsA変形細胞における細胞RNAの温度依存調節を特徴づける.
主な方法:
- SV40変異細胞からcDNAライブラリの構築とスクリーニング.
- 細胞のmRNAレベルを定量化するためのハイブリダイゼーション技術.
- RNA種とその調節を確認するための北極点分析.
主要な成果:
- 11個のcDNAクローンは,SV40変異細胞の細胞mRNA値が上昇していることを検出しました.
- 3つのクローンは,許容温度と非許容温度でSV40tsA細胞の100倍以上の増加を示しました.
- 複数のRNA種の温度依存の調節が観察されました.
- 3T3細胞のSV40感染は,補完的なRNAレベルを刺激しました.
結論:
- SV40大T抗原は,特定の細胞RNA種の安定状態レベルを直接調節する.
- この調節は,大T抗原の機能状態 (温度感受性) に依存することがあります.
- 特定された細胞RNAは,SV40媒介遺伝子調節の潜在的な標的を代表しています.
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