酵母LEU2の核酸配列は,5'-ノンコーディング領域がルシンの配列に類似していることを示しています

A Andreadis, Y P Hsu, G B Kohlhaw

Cell

|December 1, 1982

まとめ

研究者らは,酵母におけるLEU2遺伝子の規制配列を特定した. 発見は,下流のヘアピン構造を含む新しいRNAポリメラーゼIII依存の規制モデルを示唆しています.

科学分野:

分子生物学は分子生物学である.
イースト遺伝学イースト遺伝学

背景:

LEU2遺伝子は,ルシンの生物合成における重要な酵素をコードする.
遺伝子調節を理解することは,代謝工学と合成生物学にとって極めて重要です.

研究の目的:

酵母におけるLEU2遺伝子の調節配列を分離し,特徴づけること.
LEU2遺伝子の転写調節メカニズムを解明する.

主な方法:

制限酵素消化 (Xho I-Sal I) を用いた遺伝子分離.
5'-ノンコーディング領域のDNAシーケンシング.
潜在的な規制要素のバイオインフォマティック分析 (オープン・リーディングフレーム,ヘアピン構造,ターミネーター配列).

主要な成果:

LEU2遺伝子と規制配列を含む2200bpの断片を分離しました.
5'-ノンコーディング領域で23コドンの開いた読み取りフレームを特定し,酵母のようなルシンのコドンの使用が確認されました.
LEU2のコーディングシーケンスの下流で潜在的なRNAポリメラーゼターミネーターヘアピン構造を発見した.
LEU2の上流にあるtRNALeu3遺伝子を特定し,RNAポリメラーゼIIIによって転写した.

結論:

LEU2遺伝子とその調節要素は,同じDNA鎖に位置しています.
LEU2のための新しい規制モデルが提案され,RNAポリメラーゼIIIの転写と下流のmRNAヘアピン構造を含む.
これらの発見は,酵母遺伝子の調節と遺伝子操作の潜在的な標的に関する洞察を提供します.

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