関連する実験動画
Updated: May 6, 2026

11:56
Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
11.0K
まとめ
ヘルペス・シンプレックスウイルス1型 (HSV1) のグリコプロテインは,ゴルギ装置の末期にO結合グリコシル化を受けます. この変異は,彼らの電泳的運動性に大きく影響し,ウイルス性グリコプロテインの成熟に不可欠です.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- グライコバイオロジーは,
- 細胞生物学 細胞生物学
背景:
- ヘルペス・シンプレックスウイルス1型 (HSV1) のグリコプロテインは,翻訳後の広範な修正を受けます.
- これらの変化を理解することは,HSV1の病原性を理解し,抗ウイルス薬の開発の鍵です.
研究 の 目的:
- 成熟したHSV1グリコプロテインのO結合オリゴサッカライドを特徴付けるために.
- O-リンクされたグリコシル化と他の翻訳後の改変の間の時間的関係を解明する.
主な方法:
- HSV1のグリコプロテインの電泳分析.
- メタボリックラベリングとパルス追跡実験.
- モネシンとリシン耐性細胞系を用いた抑制研究.
主要な成果:
- O連結オリゴサッカライドは,成熟したHSV1グリコプロテインの低電離性移動性に大きく貢献します.
- 脂肪酸アシレーションは,O結合オリゴサッカリドの獲得に先行する.
- N-リンクされたオリゴサッカライドを複合型に変換することは,O-リンクされたグリコシル化と一致する.
結論:
- HSV1のグリコプロテインにO-リンクされたオリゴサッカライドの拡張と結合は,後期的な翻訳後のイベントとして発生します.
- このプロセスは,ゴルジ装置で起こります.
- O-リンクされたグリコシライゼーションは,N-リンクされたオリゴサッカリドの処理のための細胞機械に依存しています.
関連する概念動画
Membrane Carbohydrates
5.9K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
5.9K
Protein Glycosylation
8.5K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
8.5K
Oligosaccharide Assembly
2.7K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.7K
Proteoglycans
4.1K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
4.1K
Glycocalyx and its Functions
9.2K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
9.2K
Formation of Lipopolysaccharides
1.1K
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
1.1K

