哺乳類の染色体複製体の初期領域のクローニング
関連する実験動画
Updated: Oct 22, 2026

17:14
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
Published on: December 10, 2012
Nature
|March 6, 1983
まとめ
研究者らは,特定のDNA断片を特定し,真核細胞の複製を開始した. この発見は,繰り返されるDNA単位である各アンプリカンには,複製の単一の起源があり,DNA合成を明確にすることを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- ユカリオット染色体は数千のレプリカンを含んでおり,それぞれがS相の間に複製します.
- 生体内におけるエウカリオット染色体複製の起源を特定することは,依然として困難です.
- 証拠によると,DNA合成は特定の部位で始まります.
研究 の 目的:
- 機能性染色体起源を in vivo で特定するためのシステムを開発する.
- 複製の起源として機能する特定のDNA配列を特定するために.
主な方法:
- メソトレキサート耐性の中国ハムスター卵巣細胞系 (CHOC 400) を開発し,DHFR遺伝子を含む早期複製配列の1,000コピーを備えた.
- S相の初めの繰り返し単位 (アンプリカン) 内のDNA合成開始部位を分析した.
- 早期に標識された断片 (ELF) を識別するために分子クローンと制限マッピングを使用しました.
主要な成果:
- それぞれのアンプリカン内のDNA合成の開始は,制限断片の小さなサブセットに制限されていました.
- すべての早期に標識された断片 (ELF) は,各繰り返し単位内の単一の場所から派生しました.
- これは,アンプリカオン"人当たりの複製の単一の起源を示す.
結論:
- それぞれのアンプリカンは,S相の開始時に,複製の単一の起源からDNA合成を開始します.
- アンプリカンは機能的にはレプリカンに相当します.
- この研究は,真核生物の複製の起源を特定し,隔離するための方法を提供します.
関連する概念動画
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell’s circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from two replication forks, resulting in two DNA molecules.Many Proteins Work Together to Replicate the ChromosomeReplication is coordinated and carried out by a host of specialized proteins.
Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.Many Proteins Orchestrate Replication at the OriginEukaryotic replication follows many of the same principles...
Chromosome Structure
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Replication
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

