関連する実験動画

Updated: May 11, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

rDNAクロマチンの転写的に活発な状態と不活性な状態における核細胞構造とヒストンH3アクセシビリティの可逆的な変化

C P Prior, C R Cantor, E M Johnson

    Cell
    |October 1, 1983
    PubMed
    まとめ

    サルフヒドリルラベリングは,トランスクリプトとノントランスクリプトのPhysarum rDNA間のクロマチンの構造の違いを明らかにします. ヒストンH3の反応性は,転写された領域に特異的であり,遺伝子発現における異なる構造的役割を示している.

    さらに関連する動画

    Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers10:28

    Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

    Published on: September 20, 2018

    RNA-Associated Chromatin DNA-DNA Interaction Method11:01

    RNA-Associated Chromatin DNA-DNA Interaction Method

    Published on: April 30, 2026

    関連する概念動画

    Nucleosome Remodeling02:54

    Nucleosome Remodeling

    Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
    Nucleosome remodeling complex
    Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
    Histone Modification02:32

    Histone Modification

    The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
    Acetylation
    The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
    Spreading of Chromatin Modifications02:25

    Spreading of Chromatin Modifications

    The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
    Writers
    The writer is an enzyme that can...
    Duplication of Chromatin Structure02:05

    Duplication of Chromatin Structure

    The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
    The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
    Heterochromatin02:38

    Heterochromatin

    The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
    Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
    Histone Modification02:32

    Histone Modification

    The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
    Acetylation
    The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...