まとめ
FBJマウリン骨肉腫ウイルス (FBJ-MuSV) の腫瘍遺伝子v-fosは,細胞を変容させるタンパク質 (p55v-fos) を生成します. ウイルスと細胞の両方のフォスタンパク質は,細胞核に存在し,細胞形態はより多くの修正を受けています.
科学分野:
- 腫瘍原性ウイルス研究
- 細胞および分子生物学は,細胞および分子生物学である.
- 遺伝子発現とタンパク質の改変
背景:
- FBJマウリン骨肉腫ウイルス (FBJ-MuSV) は,マウスの骨肉腫を誘発し,線維芽細胞を変換することが知られている.
- FBJ-MuSVの腫瘍遺伝子,v-fosは,ヘルパーウイルスとの再結合によって細胞遺伝子 (c-fos) から発生します.
- v-fos遺伝子は5万5000ダルトンのタンパク質,p55v-fosをコードし,細胞の変異に関与しています.
研究 の 目的:
- 細胞内のv-fosタンパク質 (p55v-fos) とその細胞の同位体であるc-fos.の細胞の局所と特性を調査する.
- 翻訳後の改変とp55v-fosとp55c-fosの核レベルを比較するために.
- ウイルス腫瘍形成と細胞変容におけるフォスタンパク質の役割を理解する.
主な方法:
- 免疫光顕微鏡を用いて,細胞内のフォスタンパク質を検出し,局所化する.
- FBJ-MuSVに感染した細胞,またはv-fosまたはc-fosを発現する再結合プラズミッドによって変異した細胞の分析.
- タンパク質の改変レベルを評価するための生化学分析.
主要な成果:
- p55v-fosタンパク質は,増幅されたv-fos遺伝子を持つ細胞の核およびウイルスに変異した細胞で検出されました.
- 細胞のc-fosタンパク質 (p55c-fos) は,マウスの正常な乳腺細胞とc-fos遺伝子を発現する細胞の核に局所されました.
- p55c-fos.は,p55v-fos.と比較して,核におけるより広範な翻訳後の改変を示した.
- 正常細胞のp55c-fosの核濃度は,変異した線維芽細胞のp55v-fosとp55c-fosの核濃度と同等であった.
結論:
- ウイルス (v-fos) と細胞 (c-fos) の両方のタンパク質は核タンパク質である.
- 核内のc-fosの翻訳後の改変は,v-fosの改変よりも複雑である.
- 正常な細胞と変異した細胞におけるフォスタンパク質の発現レベルは,ウイルスの変異を超えた細胞プロセスにおける役割を示唆しています.
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