Jove
Visualize
お問い合わせ

関連する概念動画

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

65.9K
This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal...
65.9K
Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure08:40

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

8.0K
A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is...
8.0K
Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function11:47

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

11.1K
We present a biochemical and behavioral protocol to evaluate the efficacy of mitochondria-targeted water-soluble compounds for the treatment of Spinocerebellar ataxia type 1 (SCA1) and other cerebellar neurodegenerative diseases.
11.1K
Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain05:51

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

20.3K
Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.
20.3K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

13.9K
Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
13.9K
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

61.6K
We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to...
61.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A novel xeno-organoid approach: exploring the crosstalk between human iPSC-derived PGC-like and rat testicular cells.

Molecular human reproduction·2020
Same author

Single-cell gene expression analysis reveals diversity among human spermatogonia.

Molecular human reproduction·2017
Same author

Transcription factor - induced pluripotency.

Journal of stem cells & regenerative medicine·2014
Same author

Bcar3 is expressed in sertoli cells and germ cells of the developing testis in mice.

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation·2011
Same author

Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Laser secondary neutral mass spectrometry for copper detection in micro-scale biopsies.

Journal of mass spectrometry : JMS·2009
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
関連記事をすべて見る
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する実験動画

Updated: Jan 9, 2026

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

65.9K

強化剤を含む分子と細胞の成分との間の特定の相互作用.

H R Schöler, P Gruss

    Cell
    |February 1, 1984
    PubMed
    まとめ
    この要約は機械生成です。

    強化要素は,機能のために特定の細胞因子を必要とし,競争試験によって実証されています. これらの要因は増強剤の活性において極めて重要であり,細胞タイプ特有の増強剤の好みを説明する可能性がある.

    さらに関連する動画

    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
    11:56

    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

    Published on: April 12, 2014

    13.9K
    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
    14:55

    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

    Published on: May 12, 2013

    61.6K

    関連する実験動画

    Last Updated: Jan 9, 2026

    An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
    06:40

    An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

    Published on: March 19, 2018

    65.9K
    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
    11:56

    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

    Published on: April 12, 2014

    13.9K
    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
    14:55

    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

    Published on: May 12, 2013

    61.6K

    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝子規制 遺伝子規制
    • バイオケミストリー バイオケミストリー

    背景:

    • 強化剤は,遺伝子転写を調節する重要なDNA配列です.
    • 強化剤の機能と細胞タイプ特異性の基礎となる正確な分子機構は完全に理解されていません.
    • SV40 (Simian vacuolating virus 40) 増強剤は,増強剤の活性性を研究するためのよく特徴づけられたモデルシステムである.

    研究 の 目的:

    • 強化要素と細胞因子の間の分子相互作用を調査する.
    • 強化剤の活性に特定の細胞成分が必要かどうかを判断する.
    • 異なる強化成分に対する宿主細胞の好みの基礎を探求する.

    主な方法:

    • 増強剤-細胞成分相互作用を研究するために,インビボのコンペティションアッセイを用いた.
    • 強化剤を含む様々な分子とSV40プロモーター要素の間の競争を調査した (72 bpの繰り返し,TATAボックス).
    • SV40 72 bp リピート内のポイントミュータントが細胞因子に競争する能力を評価した.

    主要な成果:

    • 強化剤を含む分子の間の重要な細胞成分に対する競争が実証されています.
    • 強化剤は,他のSV40プロモーター要素とは異なり,これらの因子に対して競合することを示した.
    • 非機能増強剤の変異体が競合できないことを特定し,機能増強剤の役割を確認しました.
    • 異質増強剤は一般的な細胞分子に競い合うが,宿主細胞の好みは依然として明らかである.

    結論:

    • 増強剤と相互作用する細胞因子は,その機能のために必要である.
    • コンペティションアッセイは,強化剤の活性性の分子基礎についての洞察を提供します.
    • 増強剤に対する宿主細胞の好みは,これらの共有された細胞成分との異なる相互作用によって説明され得る.