関連する実験動画

Updated: Dec 10, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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正常および変異したヒトβ-グロービンプレ-mRNAは,in vitroで忠実かつ効率的にスプライスされます

A R Krainer, T Maniatis, B Ruskin

    Cell
    |April 1, 1984
    PubMed
    まとめ

    この研究は,ヒトβ-グロービンのmRNA前駆体におけるin vitro splicingがHeLa細胞核抽出物において効率的かつ正確であることを示しています. キャピングはスペライシングの特異性を高め,ベータ・タラセミアを引き起こす変異は,インビトロとインビボで同様にスペライシングに影響します.

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