エピダーマルの成長因子受容体と超巻きDNAとの間で発生するATP刺激による相互作用

B Mroczkowski, G Mosig, S Cohen

Nature

|May 17, 1984

まとめ

皮膜成長因子 (EGF) 受容体であるチロシンキナーゼは,DNAと直接相互作用し,DNAをニックすることができます. この発見は,成長因子受容体のシグナル伝達以外の新たな役割を示唆しています.

科学分野:

分子生物学は分子生物学である.
細胞シグナリング
バイオケミストリーバイオケミストリー

背景:

表皮成長因子 (EGF) 受容体は,チロシン特異のキナーゼ活性を持つトランスメブラングリコタンパク質である.
EGFの結合は受容体キナーゼの活性を高め,基板のリン酸化と自己リン酸化につながります.
インスリンと血小板由来成長因子 (PDGF) 受容体との類似性は存在し,ホルモン受容体とウイルスの変容タンパク質に共有されたチロシンキナーゼ機能があることを示唆しています.

研究の目的:

EGF受容体とDNAの潜在的相互作用を調査する.
EGF受容体内部化の機能的影響とその潜在的な核相互作用を調査する.

主な方法:

EGF受容体の浄化を行います.
DNAの相互作用とニッキング活動の測定.

主要な成果:

精製されたEGF受容体は,超巻きの二重鎖DNAと相互作用する能力を示した.
この相互作用により,ATPが刺激した方法でDNAが切り裂かれました.

結論:

EGF受容体は,これまで未知のDNA結合およびニッキング能力を有しています.
この発見は,DNAを含む細胞プロセスにおける成長因子受容体の直接的な役割を理解するための新しい道を開きます.

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