まとめ
シミアン・サーコマウイルス (v-sis) の遺伝子製品は,ヒトの血小板由来成長因子 (PDGF) に似ています. 研究者はヒトの遺伝子 (c-sis) をクローンし,PDGFポリペプチドをコードしていることが判明し,霊長類の進化の間に強力な保全を示しています.
科学分野:
- 分子生物学は分子生物学である.
- 腫瘍学 腫瘍学
- 遺伝学 遺伝学とは
背景:
- 類人猿のサルコマウイルスの変容遺伝子であるv-sisは,ヒトの血小板由来成長因子 (PDGF) と密接に関連しているp28sisというタンパク質をコードしています.
- 血小板由来成長因子 (PDGF) は,結合組織細胞にとって強力なミトゲンです.
研究 の 目的:
- v-sis. に関連するヒトの遺伝子をクローンして特徴づけること.
- ヒトの遺伝子がPDGFポリペプチドをコードしているかどうかを判断する.
主な方法:
- ヒトのV-SISに関連した場所のクローニング.
- ヒト遺伝子のエクソンの核酸配列解析.
- 予測されたヒトc-sisアミノ酸配列とv-sisおよびPDGF配列の比較.
主要な成果:
- 人間のv-sis関連ロカスには,少なくとも5つのエクソンが含まれています.
- 人間のc-sisの予測されたアミノ酸配列は,v-sisと6%異なっている.
- 人間のc-sisは主要なPDGFポリペプチドの構造遺伝子である.
結論:
- シス・プロト・オンコゲンは,霊長類の進化の過程でよく保存されている.
- 人間のc-sis遺伝子は,重要な細胞成長調節体であるPDGFの機能成分をコードする.
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