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Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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関連する実験動画

Updated: Jun 23, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

酵母染色体複製器の機能に関する構造要求事項

S Kearsey

    Cell
    |May 1, 1984
    PubMed
    まとめ

    研究者は,酵母における自律的な複製に不可欠な重要な14bpのコア領域を特定しました. この領域は,Saccharomyces cerevisiae HO遺伝子の近くにあり,DNA複製の起源機能に不可欠です.

    科学分野:

    • 分子生物学は分子生物学である.
    • イースト遺伝学 イースト遺伝学
    • DNAレプリケーション DNA複製

    背景:

    • Saccharomyces cerevisiaeのHO遺伝子は,酵母における自律的な複製を可能にする配列と関連付けられています.
    • 複製起源の構造的要件を理解することは,DNA複製メカニズムを理解するための鍵です.

    研究 の 目的:

    • 酵母複製の起源を推測する重要な構造要素を特定する.
    • In vivoの自律的な複製のための特定のDNA配列の機能的重要性を決定する.

    主な方法:

    • 消去型変異は,DNA配列のセグメントを削除するために使用されました.
    • ポイント変異は,配列内の特定のヌクレオチドを変更するために使用されました.
    • 機能分析により,酵母における自律的な複製が評価されました.

    主要な成果:

    • 14pbのコア領域は,自律的な複製に不可欠であると特定されました.
    • このコア領域内の点変異は,複製を廃止しました.
    • 隣接する20bpの配列は複製効率を高めましたが,厳格に必要ではありませんでした.

    結論:

    さらに関連する動画

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

    関連する実験動画

    Last Updated: Jun 23, 2026

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
    06:40

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

    Published on: March 22, 2018

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

    • 14bpのコア配列は,酵母における自律的な複製起源機能の決定的な決定因子である.
    • コア領域を横断する配列要素は,複製効率に寄与するが,その効率化には必要ではない.