まとめ
研究者たちは,遺伝子移植を用いてヒトのトランスファーリン受容体遺伝子をクローン化しました. 彼らは受容体エンコーディングに十分な31kbのDNA断片を特定し,そのエクソンと翻訳されていない領域を特徴付けました.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- トランスファーリン受容体は,細胞の鉄吸収に不可欠です.
- その遺伝子構造を理解することは,細胞の鉄代謝の研究の鍵です.
研究 の 目的:
- ヒトのトランスファーリン受容体遺伝子の分子クローニングを達成するために.
- その発現に必要なDNA配列を特定するために.
主な方法:
- ネズミのLtk-細胞に遺伝子移植.
- ヒポキサンチン/アミノプテリン/チミジン (HAT) 媒介を用いた選択.
- 光活性化された細胞分類 (FACS).
- ゲノムとcDNA図書館のスクリーニング.
主要な成果:
- トランスファーリン受容体遺伝子をコードする34kbのヒトDNAを分離した.
- L細胞における受容体発現に31kbのDNAが十分であることを決定した.
- 遺伝子内の少なくとも12のエクソンを特定しました.
- 4.9kbのcDNAと2kbの3'未翻訳配列を特徴づけました.
結論:
- ヒトのトランスファーリン受容体遺伝子をクローンし,特徴づけることに成功した.
- 受容体発現に必要な最小のDNA領域を定義した.
- 遺伝子構造と規制要素についての洞察を提供した.
関連する概念動画
09:10Measuring Single-Cell Aging with an Imaging-based Biomarker of Chromatin and Epigenetic Aging
208
The protocol presents the imaging and computational workflow to extract and validate imaging-based chromatin and epigenetic age...
208
04:40Electrostatic Method to Remove Particulate Organic Matter from Soil
5.2K
Removing recently deposited and incompletely decomposed plant material from soil samples reduces the influence of temporary seasonal inputs on soil organic carbon measurements. Attraction to an electrostatically charged surface can be used to quickly remove a substantial amount of particulate organic...
5.2K
08:57Improving Infrared Spectroscopy Characterization of Soil Organic Matter with Spectral Subtractions
13.2K
SOM underlies many soil functions and processes, but its characterization by FTIR spectroscopy is often challenged by mineral interferences. The described method can increase the utility of SOM analysis by FTIR spectroscopy by subtracting mineral interferences in soil spectra using empirically obtained mineral reference...
13.2K
13:29Production and Measurement of Organic Particulate Matter in a Flow Tube Reactor
8.0K
This paper describes the operation procedure for the flow tube reactor and related data collection. It shows the protocols for setting the experiments, recording data and generating the number-diameter distribution as well as the particle mass information, which gives useful information about chemical and physical properties of the organic...
8.0K
09:46Production and Measurement of Organic Particulate Matter in the Harvard Environmental Chamber
7.7K
This paper describes operation procedures for the Harvard Environmental Chamber (HEC) and related instrumentation for measuring gaseous and particle species. The environmental chamber is used to produce and study secondary organic species produced from the organic precursors, especially related to atmospheric organic particulate matter.
7.7K
09:38Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems
11.0K
Dissolved organic matter provides an important source of energy and nutrients to stream ecosystems. Here we demonstrate a field-based method to manipulate the ambient pool of dissolved organic matter in situ through easily replicable nutrient...
11.0K


