IS50の直接的な繰り返しの間のRECA独立した再結合です

T J Zupancic, S L Marvo, J H Chung

Cell

|June 1, 1983

まとめ

Tn5の隣接するIS50配列の再組み合わせは,E. coliで容易に発生します. この再結合にはIS50転置タンパク質も必要であり,同類再結合に似ています.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
バクテリアの転移

背景:

トランポゾンはTn5と同様に,その運動と調節のために挿入配列 (IS) を利用します.
IS50は,Tn5の横に並ぶ挿入シーケンスであり,トランポジションに不可欠な直接繰り返しを含んでいます.
細菌における再結合メカニズムは,遺伝的多様性とゲノム安定性にとって不可欠である.

研究の目的:

IS50配列の直接的な繰り返しの間の再結合プロセスを調査する.
この再結合におけるIS50でコードされたポリペプチドの役割を決定する.
IS50媒介による再結合の背後にあるメカニズムを解明する.

主な方法:

E. coli recA菌株を直接IS50を含むプラズミドで変換する.
IS50変異体の分析により,その暗号化されたポリペプチドの機能を評価する.
遺伝子解析による再結合メカニズムの特徴化.

主要な成果:

IS50の直接繰り返しの間の再結合は,E. coli recA株で容易に観察されました.
転置に不可欠なIS50でコードされたポリペプチドは,IS50再結合にも必要である.
リコンビネーションメカニズムは,サイト特異ではなく,一般的な同類リコンビネーションであるようです.

結論:

IS50媒介による再結合は,転置タンパク質によって促進されます.
この再結合システムは,IS50とTn5のトランスポーゼーション中にコインテグレート構造を解決する可能性が高い.
この発見は,転置と同類再結合経路の間の関連性を示唆しています.

さらに関連する動画

08:17

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Published on: February 15, 2019

39.5K

14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.8K

関連する概念動画

02:03

Law of Independent Assortment

62.7K

While Mendel’s Law of Segregation states that the two alleles for one gene are separated into different gametes, a different question of how different genes are inherited remains. For example, is the gene for tall plants inherited with the gene for green peas? Mendel asked this question by experimenting with a dihybrid cross; a cross in which both parents are homozygous for two distinct traits resulting in an F1 generation that are heterozygous for both traits.

62.7K

02:31

Homologous Recombination

63.0K

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

63.0K

01:21

Introduction to Test of Independence

3.0K

In statistics, the term independence means that one can directly obtain the probability of any event involving both variables by multiplying their individual probabilities. Tests of independence are chi-square tests involving the use of a contingency table of observed (data) values.
The test statistic for a test of independence is similar to that of a goodness-of-fit test:

3.0K

01:16

Hypothesis Test for Test of Independence

8.2K

The test of independence is a chi-square-based test used to determine whether two variables or factors are independent or dependent. This hypothesis test is used to examine the independence of the variables. One can construct two qualitative survey questions or experiments based on the variables in a contingency table. The goal is to see if the two variables are unrelated (independent) or related (dependent). The null and alternative hypotheses for this test are:
H0: The two variables (factors)...

Recombinant DNA

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.