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関連する概念動画

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

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関連する実験動画

Updated: Jun 17, 2026

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
13:20

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Published on: July 30, 2010

免疫グロブリン遺伝子転写は,下流配列要素によって活性化されます.

C Queen, D Baltimore

    Cell
    |July 1, 1983
    PubMed
    まとめ

    研究者らは,カッパ免疫グロブリン遺伝子発現の調節に不可欠なDNA配列を特定した. これらの配列は,J・カッパ領域の下流に位置し,骨髄腫細胞における正確な遺伝子転写に不可欠である.

    科学分野:

    • 免疫学 免疫学とは
    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは

    背景:

    • カッパの免疫グロブリン遺伝子発現は厳しく規制されています.
    • 調節性DNA配列を理解することは,抗体産生を制御する鍵です.

    研究 の 目的:

    • カッパ免疫グロブリン遺伝子発現を調節するDNA配列を特定し,特徴づけること.
    • カッパ遺伝子転写における下流配列の役割を決定する.

    主な方法:

    • カッパ遺伝子の転写を検出するための一時的な発現アッセイの開発.
    • クローン化され,再編成されたカッパ遺伝子をマウス骨髄腫とサル細胞系に転移させる.
    • 規制要素を特定するために,カッパ遺伝子の削除分析.

    主要な成果:

    • トランスクリプションは,骨髄腫細胞のカッパ遺伝子の再配置から検出されました.
    • ダウンストリーム配列の削除は,骨髄腫細胞の転写を廃止し,その必要性を示しました.
    • トランスクリプションは猿の細胞でも検出されましたが,下流配列より低く,独立したものでした.

    結論:

    さらに関連する動画

    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
    13:55

    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

    Published on: February 3, 2013

    Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
    12:54

    Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

    Published on: March 7, 2018

    関連する実験動画

    Last Updated: Jun 17, 2026

    Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
    13:20

    Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

    Published on: July 30, 2010

    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
    13:55

    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

    Published on: February 3, 2013

    Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
    12:54

    Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

    Published on: March 7, 2018

  • カッパ変数領域プロモーターの下流のDNA配列は,骨髄腫細胞の正確な転写開始に不可欠です.
  • これらの下流配列は,カッパ免疫グロブリン遺伝子発現を調節する上で重要な役割を果たします.