ヒトの慢性リンパ球性白血病と相関する細胞表面抗原の発現に責任を負う機能的な遺伝子のクローン化
Cell
|November 1, 1981
まとめ
研究者らは,ハイブリッド細胞系を用いて慢性リンパ球性白血病細胞に新しい表面抗原を特定した. この抗原は,中国ハムスター卵巣 (CHO) とヒトリンパ細胞のハイブリッドによって生成され,白血病細胞検出のための潜在的な標的を提供します.
科学分野:
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- 中国ハムスター卵巣 (CHO) 細胞とヒトB細胞慢性リンパ球性白血病 (CLL) リンパ芽細胞を融合させ,ハイブリッド細胞系が作られた.
- このハイブリッド細胞系は,ユニークな表面抗原を表現する.
研究 の 目的:
- 人間とCHOのハイブリッド細胞系によって生産された新しい表面抗原を特徴付ける.
- この抗原が慢性リンパ球性白血病 (CLL) 細胞に特異性があるかどうかを判断する.
主な方法:
- CHO細胞とヒトCLLリンパ球の間の細胞-細胞ハイブリッドの生成.
- 機能的な遺伝子を分離するために,ハイブリッド細胞から遺伝子ライブラリをスクリーニングする.
- ヒト特異的な繰り返し配列と標的表面抗原を含むクローンの識別.
主要な成果:
- 新種の表面抗原は,CLL細胞の一部に検出されたが,正常リンパ球には検出されなかった.
- 抗原を発現する2つの再結合クローンが,ハイブリッド細胞の遺伝子ライブラリから分離されました.
- これらのクローンには,ヒトとCHO固有のDNA配列の両方が含まれていた.
結論:
- この研究では,CLL特異的な表面抗原を生成する遺伝子を成功裏に特定し,隔離しました.
- 研究結果は,抗原が慢性リンパ球性白血病のバイオマーカーとして機能する可能性があることを示唆しています.
- ハイブリッド細胞技術は,混合ゲノム内の特定のヒト遺伝子の分離を容易にした.
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