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発達中のヒトの胎盤における細胞性腫瘍遺伝子の発現の空間的および時間的なパターン:胚細胞増殖への影響

S Pfeifer-Ohlsson, A S Goustin, J Rydnert

|September 1, 1984

まとめ

人間のc-myc遺伝子は

科学分野:

分子生物学は分子生物学である.
発達生物学発達生物学とは
遺伝学遺伝学とは

背景:

c-myc遺伝子は,細胞の成長と分化に関与する原発がん遺伝子である.
人間の正常な胎盤発達におけるその役割は完全に理解されていません.

研究の目的:

人間の胎盤発達中のc-myc遺伝子の発現パターンと機能を調査する.

主な方法:

ノーザン・ブロット,ドット・ブロット,イン・シトゥ・ハイブリダイゼーションを用いた段階的なヒトの胎盤の分析.
c-mycトランスクリプトレベルの定量化.
胎盤組織内のc-mycトランスクリプトの局所化.

主要な成果:

胎盤RNAでステージ固有の2.4kbのc-mycトランスクリプトが特定され,受胎後4〜5週間でピーク発現しました.
c-mycトランスクリプトのレベルは胎盤発達の過程で著しく変化し,第1 trimesterの終わりまでに減少しました.
In situ ハイブリダイゼーションでは,胎盤の早期の細胞ホルモンの殻で高いc-myc発現を明らかにしました.
c-mycトランスクリプトの豊富性と細胞ホルボブラストの増殖の間に強い相関が観察されました.

結論:

c-myc遺伝子は,ヒトの胎盤発達の初期に細胞トロフォブラストの増殖を調節する上で重要な役割を果たします.
c-mycの発現は,発達中の胎盤内で厳格に調節され,空間的に局在しています.

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