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Updated: Jun 11, 2026

10:14
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Published on: October 23, 2010
まとめ
C6VL Tリンパ腫細胞受容体は,二硫化物結合ヘテロダイマーであり,迅速な組み立てと翻訳後の広範なグリコシライゼーションを経験します. そのクローン型エピトープと抗原結合部位は,受容体組み立て後に現れる可能性があります.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- C6VLTリンパ腫細胞の抗原特異受容体はヘテロジマーである.
- この受容体は,電荷微異質性を持つアルファとベータ鎖で構成されています.
研究 の 目的:
- C6VLTリンパ腫細胞受容体の合成と翻訳後の改変を調査する.
- エピトープ発現のタイミングと,組み立て後のイベントへの潜在的な依存を決定する.
主な方法:
- タンパク質合成とエピトープ検出を追跡するためのパルスチェイスラベリング実験.
- エンドグリコシダゼHとFの消化により,N結合オリゴサッカリド鎖を分析する.
- 脱糖化前のおよび後のサブユニット分子重量の分析.
主要な成果:
- この受容体は,39kdα鎖と41kdβ鎖の二硫化物結合ヘテロダイマーである.
- 抗受容体エピトープは早期に (2分) 検出されたが,クローン型エピトープは後に (10分) 出現した.
- 両方のサブユニットは,少なくとも3つのN結合オリゴサッカリド鎖を有し,脱糖化された形態は27kd (α) と32kd (β) にあります.
結論:
- 二次受容体は,翻訳後に急速に形成され,その後に広範なグリコシル化が行われます.
- クローン型エピトープの出現と抗原結合部位の可能性は,組み立て後の修正に依存している可能性が高い.
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関連する概念動画
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Antigen Presenting Cells
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
Antigen Processing Pathways
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

