関連する実験動画

Updated: Jun 17, 2026

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

mRNA前駆体のインビトロスプライシングにおけるキャップ構造の認識

M M Konarska, R A Padgett, P A Sharp

    Cell
    |October 1, 1984
    PubMed
    まとめ

    細胞抽出物における効率的なRNAスプライシングには,5'キャップ構造が必要です. Cap アナログはスプライシングを阻害し,cap認識が必須のリボ核タンパク質複合体の形成に不可欠であることを示唆しています.

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    RNA Splicing01:32

    RNA Splicing

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    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
    RNA Splicing01:32

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    Alternative RNA Splicing02:18

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    Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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