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The Extracellular Matrix01:42

The Extracellular Matrix

Overview
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...

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Updated: May 11, 2026

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

細胞間LETSグリコタンパク質マトリックスに関する研究

L B Chen, A Murray, R A Segal

    Cell
    |June 1, 1978
    PubMed
    まとめ
    この要約は機械生成です。

    チッキーの胚性線維芽細胞は,主にLETS (Large External Transformation Sensitive) タンパク質で構成される細胞外マトリクスを分泌する. ディスルファイド結合で結合されたこれらのLETSタンパク質マトリクスは,細胞の形状とミオブラスト融合に影響を与えます.

    さらに関連する動画

    Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
    14:02

    Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

    Published on: April 21, 2017

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
    11:21

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

    Published on: January 20, 2022

    関連する実験動画

    Last Updated: May 11, 2026

    A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
    09:40

    A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

    Published on: January 4, 2017

    Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
    14:02

    Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

    Published on: April 21, 2017

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
    11:21

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

    Published on: January 20, 2022

    科学分野:

    • 細胞生物学 細胞生物学
    • バイオケミストリー バイオケミストリー
    • 細胞外マトリックス研究

    背景:

    • 細胞と細胞の接触は,鶏の胚性線維芽細胞による細胞外マトリックス形成に不可欠である.
    • これらの行列は繊維状で,個々の繊維の直径は5〜10nmで,それらは束に集約されます.

    研究 の 目的:

    • 鶏の胚性線維芽細胞によって分泌される細胞間マトリクスの組成と性質を調査する.
    • マトリックス形成と機能におけるLETS (Large External Transformation Sensitive) タンパク質の役割を決定する.

    主な方法:

    • スキャン・トランスミッション電子顕微鏡で,マトリックス構造を可視化します.
    • タンパク質の成分を特定するための免疫光と免疫オフェリチン技術.
    • セルフリーマトリクスを分離するために洗剤処理.
    • ベータ-メルカプトエタノールを用いた溶解化研究.

    主要な成果:

    • LETSタンパク質は主要な成分であり,細胞フリーマトリックスの90%以上を占めています.
    • マトリクスは,溶解要求によって示されるように,二硫化結合で結びついているLETSタンパク質のポリマーである可能性が高い.
    • コラーゲンはこれらのマトリックスには含まれていません.
    • LETSタンパク質マトリクスは,細胞の形状変化とミオブラスト融合を促進しますが,細胞の成長,グルコースの吸収,またはプロテアゼの生成に影響しません.

    結論:

    • チップの胚繊維芽細胞の細胞外マトリクスは主にLETSタンパク質で構成され,二硫化物結合ポリマーを形成します.
    • LETSタンパク質マトリクスは,細胞の成長や代謝活動とは関係なく,細胞形態学と融合の調節に重要な役割を果たします.