関連する実験動画

Updated: May 12, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

非繊維構造の中間性フィラメントタンパク質: 暫定的な分解と亜単位タンパク質の粒状集積物に含まれる

W W Franke, E Schmid, C Grund

    Cell
    |August 1, 1982
    PubMed
    まとめ

    ミトーシス過程で,腎臓とHeLa細胞の中間繊維は糸に解き放ち,タンパク質集積を形成します. これらの構造は,テロフェーズでフィラメントに再編成されます.

    さらに関連する動画

    In Vitro Polymerization of F-actin on Early Endosomes12:15

    In Vitro Polymerization of F-actin on Early Endosomes

    Published on: August 28, 2017

    Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy14:23

    Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

    Published on: March 6, 2018

    関連する概念動画

    Adaptability of Cytoskeletal Filaments01:12

    Adaptability of Cytoskeletal Filaments

    The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
    Assembly of Cytoskeletal Filaments01:18

    Assembly of Cytoskeletal Filaments

    Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
    The Structure of Intermediate Filaments01:19

    The Structure of Intermediate Filaments

    The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
    Intermediate filaments...
    Types of Intermediate Filaments01:31

    Types of Intermediate Filaments

    The intermediate filaments are an essential component of the cytoskeleton. Presently six types of intermediate filament have been identified. Type I and II are acidic and basic keratin proteins. Type III is of mesodermal origin and comprises four proteins: vimentin, desmin, glial fibrillary acidic protein (GFAP), and peripherin. Vimentin is commonly found in mesenchymal cells, desmin in muscle cells, GFAP in astrocytes, while peripherin is found in peripheral nervous system neurons (PNS). Type...
    Formation of Intermediate Filaments00:57

    Formation of Intermediate Filaments

    Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.
    Disassembly of Intermediate Filaments01:35

    Disassembly of Intermediate Filaments

    Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
    Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...