まとめ
マウス免疫グロブリン重鎖遺伝子組織は,複数の変数領域 (VH) 遺伝子を明らかにします. リンパ球分化中の遺伝子再編成は,特にC mu遺伝子の削除を含み,抗体クラス発現のスイッチを容易にする可能性があります.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- 免疫グロブリンの重鎖遺伝子組織を理解することは,抗体多様性とクラス切り替えの解読に不可欠です.
- 以前の研究では,哺乳類における免疫グロブリン遺伝子ロシの複雑さを特徴付け始めた.
研究 の 目的:
- 常数 (C) と変数領域 (VH) の遺伝子を含むマウス免疫グロブリン重鎖遺伝子の組織を調査する.
- 様々なB細胞悪性腫瘍 (プラズマサイトーマ) と胚性DNAにおける遺伝子文脈と再編成を調べる.
- リンパ球の微分化中に免疫グロブリンクラスの切り替えの背後にあるメカニズムを探求する.
主な方法:
- DNAの制限エンドヌクレアゼ断片を併用したクローンされたmuとalphacDNAプローブのハイブリッド化.
- BALB/c胚,肝臓,および9つのプラズマサイトマにおける遺伝子組織の比較分析.
- 特定のプローブ (HPC 76とS107) を使用した変異領域 (VH) 遺伝子サブセットの検出と比較.
主要な成果:
- ゲルムラインDNAには,単一のC mu遺伝子コピーが含まれており,複数の,異なるVH遺伝子セット (少なくとも6および4が特定されています) が含まれています.
- ほとんどのVH遺伝子は,プラズマサイトマの生殖線構成のままであり,限られた体的再編成を示唆しています.
- プラズマサイトマはしばしばC mu配列,特にIgGまたはIgAを発現するC mu配列の欠損を示しますが,C α配列は保持されます.
結論:
- ネズミの免疫グロブリン重鎖ロカスには,異なるサブセットに編成された複数のVH遺伝子があります.
- 活発な重鎖遺伝子発現のための転位イベントには,DNAの消去が含まれる可能性があります.
- C mu配列の削除は,B細胞の分化中にIgMから他の免疫グロブリンクラス (IgA,IgG) への切り替えを促進する重要なメカニズムであるようです.
さらに関連する動画
関連する概念動画
RNA Splicing
53.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
53.5K
Viral Recombination
22.3K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
22.3K
Conservative Site-specific Recombination and Phase Variation
5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.7K
Exon Recombination
3.1K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.1K
Diversity of Antigen Receptors
2.2K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
2.2K
B Cell Activation and Differentiation
14.6K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
14.6K


