膜に結合したおよび分泌されたIgM分子の免疫グロブリンミュー鎖は,それらのC末端セグメントによって異なる
Cell
|September 1, 1980
まとめ
B型リンパ球は,膜結合型と分泌型の2種類の免疫グロブリンM (IgM) を生成する. タンパク質分析により,これらのIgM重鎖は,C末端のセグメントにおいてのみ異なっていることが明らかになり,B細胞の発達と免疫に影響を与えます.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- タンパク質化学 タンパク質化学
背景:
- B型リンパ球は,発達過程で,異なる形態の免疫グロブリンM (IgM) を合成する.
- 膜に結合したIgM (mIgM) はB細胞受容体として作用し,分泌されたIgM (sIgM) は humoral免疫に不可欠である.
- ミュ・ヘビー・チェーンにおける構造的変化が,mIgMとsIgMの機能的違いを決定する.
研究 の 目的:
- 分泌されたム (μsecreted) と膜に結合したム (μmembrane) の重鎖のIgMを構造的に比較する.
- mIgMとsIgMの異なる機能の分子基盤を解明する.
主な方法:
- ペプチドマッピング
- マイクロシーケンス分析
- カーボキシペプチダゼ分析
- μmとμsのmRNAとCμ遺伝子の分析
主要な成果:
- μm と μs 鎖は,VH,Cμ1,Cμ2,Cμ3,Cμ4 ドメインで高い構造的類似性を示しています.
- 主要な構造的差異は,μm (41残留) とμs (20残留) 鎖のC端セグメントにある.
- μmのC末端セグメントの予測されたアミノ酸配列は,実験結果と一致しています.
結論:
- μm 鎖と μs 鎖はほとんど同じで,主にC端末の配列で異なっている.
- これらのC端末の変異は,B細胞機能と体液免疫における膜結合および分泌IgMの異なる役割の鍵である.
- 発見はmRNAと遺伝子分析と一致し,C端末の微分処理を裏付けている.
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