核プラズミンの核の中への移行を指定するポリペプチドドメイン

C Dingwall, S V Sharnick, R A Laskey

Cell

|September 1, 1982

まとめ

核プラズミンは,核プラズミンの

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
Xenopus laevisの卵細胞の研究について

背景:

核プラズミンは,Xenopus laevisの卵細胞で最も豊富に存在する核タンパク質です.
細胞プラズマ注入後の急速な核入りは,活発な輸送機構を示唆しています.
核プラズミンの核部位を把握することは,核輸送規制を理解する上で鍵となる.

研究の目的:

核インポートに責任を負う核プラズミンの構造領域を調査する.
核輸送における核プラズミンの尾と核ドメインの役割を決定する.
核プラズミンの選択的核蓄積のメカニズムを解明する.

主な方法:

核プラズミンペンタメールの部分タンパク質分解により,尾とコアドメインを分離する.
完ぺきな核プラズミン,ペンタメリク・コア,そして尾の領域をXenopusの卵細胞に微量注入する.
卵細胞の細胞質と核内のタンパク質の局所化の観察と定量化.

主要な成果:

核プラズミンのペンタメリク・コアには尾がなく,核に入れない.
ペンタメアの核輸送には,単一の尾部領域が十分である.
モノメアの尾は原子核に急速に蓄積し,選択的輸入に十分な尾であることを示しています.
核の侵入には尾が必要ですが,核内に留まるには尾は必要ではありません.

結論:

核プラズミンの尾領域は,Xenopusの卵細胞における選択的核輸入を媒介する.
核の侵入は尾の存在に依存し,保持は独立しています.
これらの発見は,タンパク質の核輸送を制御する分子機構の洞察を提供します.

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