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Updated: Aug 19, 2026

10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
まとめ
変異した細胞によって分泌される特定のフォスフォプロテインは,成長だけでなく,がんと関連しています. このタンパク質マーカーは,上皮組織と線維芽細胞の両方の組織における腫瘍性細胞と正常な細胞または新芽細胞を効果的に区別します.
科学分野:
- 腫瘍学 腫瘍学
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- ウイルスの細胞と自発的な細胞の両方,変換された細胞は,主要な変換に関連するフォスフォタンパク質を分泌します.
- この32Pで標識されたタンパク質の細胞外レベルは,指数関数的な成長だけでなく,変換と相関しています.
- このフォスフォタンパク質は,p60srcやシミアンウイルス40 (SV40) T抗原のような既知のウイルス抗原とは異なる.
研究 の 目的:
- この32Pラベル付フォスフォプロテインの分泌を正常および変形した上皮細胞で調査する.
- このフォスフォプロテインが,新生性腫瘍を新生性および正常な上皮細胞から区別するためのマーカーとして機能できるかどうかを判断する.
- このフォスフォタンパク質が,上皮細胞と線維芽細胞の両方の起源の腫瘍発生細胞の一般的なマーカーとしての有用性を評価する.
主な方法:
- 32Pで標識されたフォスフォタンパク質の分泌のための正常および変形した上皮細胞の比較.
- ネズミの乳頭上皮,豚の胆管上皮,ネズミの肝上皮におけるフォスフォタンパク質分泌の分析.
- フォスフォタンパク質とSV40の非ウイルス性T抗原 (pp 53) の区別は,電泳的運動性に基づいています.
主要な成果:
- フォスフォタンパク質の分泌は,ノイオプラスティックマウスの乳腺上皮を,ネネオプラスティックおよび正常な組織から区別する.
- このマーカーによって,コウモリの胆管とネズミの肝臓の表皮質からの高度に腫瘍発生性細胞が特定されました.
- フォスフォタンパク質は,p60src,p60c-src,またはSV40 T抗原と抗原的に関連していない.
結論:
- この主要なフォスフォタンパク質は,腫瘍発生細胞を特定するための信頼できるマーカーです.
- それは,正常なまたはプレネoplastic上皮組織組織から腫瘍を区別するための貴重な診断ツールとして機能します.
- リンパタンパク質が,上皮細胞と線維芽細胞の両方の起源の腫瘍発生細胞を識別する能力は,がん研究におけるその広範な重要性を強調しています.
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