細胞腫瘍遺伝子は,慢性骨髄性白血病のフィラデルフィア染色体に移位する

A de Klein, A G van Kessel, G Grosveld

Nature

|December 23, 1982

まとめ

慢性骨髄性白血病 (CML) の場合,細胞アベルソンマウリン白血病ウイルス (c-abl) 遺伝子は染色体9から染色体22に転位する. この相互の染色体交換は,CMLの発達にc-ablを伴う.

科学分野:

分子生物学は分子生物学である.
ヒューマン・ジェネティクスヒューマン・ジェネティックス
腫瘍学腫瘍学

背景:

腫瘍性レトロウイルスは,細胞性腫瘍遺伝子の同類である変異遺伝子を有する.
人間のc-abl遺伝子は,アベルソンのマウリン白血病ウイルス変異配列の同型であり,染色体9に宿っている.
フィラデルフィア染色体トランスロケーション,t(9;22) は,慢性筋細胞性白血病 (CML) の特徴です.

研究の目的:

c-abl遺伝子がCMLにおけるフィラデルフィア染色体の転位に関与しているかどうかを判断する.
トランスロケーションに関するc-ablの正確な染色体位置を調査する.

主な方法:

c-ablとv-ablのハイブリッド化プローブを使用しました.
フィラデルフィアトランスロケーションの誘導体を含む体細胞ハイブリッドを分析した.
染色体異常に対する遺伝子の位置をマッピングするために,ハイブリダイゼーションパターンを調べました.

主要な成果:

22q-誘導体 (フィラデルフィア染色体) との正交配が観察されました.
トランスロケーションの9q+誘導体で正のハイブリド化が発生しなかった.
c-abl配列がトランスロケーションされた染色体22に存在することを実証しました.

結論:

c-abl遺伝子は,CML患者において,染色体9から染色体22に転位すると結論付けました.
この発見は,相互の染色体交換の直接的な証拠を提供します.
これは,c-abl遺伝子がCMLの病原性において重要な役割を果たしていることを示唆している.

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