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Updated: May 12, 2026

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
04:49

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Published on: September 2, 2008

ウラシルの動物細胞DNAへの組み込みについて in vitro

R H Grafstrom, B Y Tseng, M Goulian

    Cell
    |September 1, 1978
    PubMed
    まとめ

    DNA合成における高レベルのデオキシウリジン三リン酸 (dUTP) は,純DNA生成を減少させる. ウラシルの組み込みは,より大きなDNA断片の合成ではなく,DNA修復と分解につながります.

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    In Vitro Reassociation Assay to Measure the Formation of 80S Ribosomal Particles Using Salt-washed Ribosomal Subunits06:09

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    Genomic DNA in Eukaryotes00:58

    Genomic DNA in Eukaryotes

    Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
    In-vitro Mutagenesis01:16

    In-vitro Mutagenesis

    To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
    In vitro Mutagenesis01:16

    In vitro Mutagenesis

    To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.