関連する実験動画

Updated: Jul 25, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

E. coli のリボソームタンパク質-RNAポリメラーゼオペロンにおける転写後の調節性変異体

N P Fiil, J D Friesen, W L Downing

    Cell
    |April 1, 1980
    PubMed
    まとめ

    rplJオペロンリーダー配列の変異は,L10リボソームタンパク質の翻訳を阻害し,高複製数のプラズミッドによって引き起こされる成長欠陥を克服します. これらの発見は,新しいRNA規制領域を特定します.

    さらに関連する動画

    Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli10:34

    Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

    Published on: August 22, 2017

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli07:04

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

    Published on: February 5, 2019

    関連する概念動画

    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
    Transcription of prokaryotic...
    Transcription Attenuation in Prokaryotes02:42

    Transcription Attenuation in Prokaryotes

    Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
    There are several different mechanisms used to attenuate transcription. In ribosome mediated...
    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
    Transcription of prokaryotic...
    Repressible Operon: trp Operon01:21

    Repressible Operon: trp Operon

    The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
    Transcriptional Regulation: Riboswitches01:23

    Transcriptional Regulation: Riboswitches

    Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
    Stringent Response in E. coli01:23

    Stringent Response in E. coli

    Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...