関連する実験動画

Updated: May 11, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 30, 2010

配列特異的な複製終端でDNA複製のインビトロ終了

J Germino, D Bastia

    Cell
    |March 1, 1981
    PubMed
    まとめ

    R6Kプラズミドの複製終端は,in vivoプロセスと同様に,DNA複製フォークをin vitroで停止します. この終結は,膜結合やプラズミドでコードされた因子を必要とせず,宿主染色体の関与を示唆します.

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    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique07:18

    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

    Published on: October 27, 2011

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome06:40

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

    Published on: March 22, 2018

    関連する概念動画

    Replication in Prokaryotes02:35

    Replication in Prokaryotes

    Overview
    Replication in Prokaryotes02:35

    Replication in Prokaryotes

    Overview
    The DNA Replication Fork01:02

    The DNA Replication Fork

    An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
    Homologous Recombination02:31

    Homologous Recombination

    The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
    Restarting Stalled Replication Forks02:37

    Restarting Stalled Replication Forks

    DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
    Replication in Prokaryotes01:32

    Replication in Prokaryotes

    DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
    Many Proteins Work Together to Replicate the Chromosome
    Replication is coordinated and carried out by a host of specialized...