酵母ヒストンmRNAの細胞周期調節

L M Hereford, M A Osley, T R Ludwig

Cell

|May 1, 1981

まとめ

酵母におけるヒストンのmRNAレベルは,細胞サイクル中に厳密に制御され,S相の初期にピークに達し,DNA合成の阻害により急速に劣化します. この規則は,細胞分裂の調整を保証する.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

ヒストンはDNAの包装と複製に不可欠です.
ヒストンのmRNA調節を理解することは,細胞周期制御を理解する鍵です.

研究の目的:

酵母におけるヒストンmRNA (H2A,H2B,H3,H4) の細胞サイクル依存的発現を調査する.
ヒストンのmRNAの蓄積とDNAの合成の関係を決定する.

主な方法:

遠心分離とアルファ因子を用いた酵母細胞同期.
ヒストンのmRNAレベルをハイブリデーションとインビトロトランスレーションによる定量化.
mRNA前駆体蓄積の分析. mRNA前駆体蓄積の分析. mRNA前駆体蓄積の分析. mRNA前駆体蓄積の分析. mRNA前駆体蓄積の分析. mRNA前駆体蓄積の分析.

主要な成果:

ヒストンのmRNAは主にS相細胞で検出される.
ヒストンのmRNAの蓄積は最大DNA合成に先行し,S相の終わりまでに減少する.
DNA合成の阻害は,正常の半減期よりも短いヒストンのmRNAの急速な分解を引き起こします.

結論:

酵母におけるヒストンのmRNAレベルは,細胞分裂中の転写および転写後のレベルで厳格に規制されています.
ヒストンのmRNA調節はDNA複製と連携している.
両方の酵母H2B遺伝子は,細胞サイクルに依存した方法で発現および調節されます.

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