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Dissection of Hippocampal Dentate Gyrus from Adult Mouse
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ウサギのアルファグロービンメッセンジャーRNAの3'端末三分の1の核酸配列:ヒトのアルファグロービンメッセンジャーRNAとの比較
Cell
|August 1, 1977
まとめ
研究者はウサギとヒトのアルファグロービンmRNAを配列化し,非コーディング領域の80%の同質性を明らかにしました. また,ヒトのアルファ・グロービンmRNAに24ヌクレオチドが挿入されていて,ベータ・グロービンmRNAで見つかったものと類似しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- アルファグロービンmRNAは,ヘモグロビン合成において重要な役割を果たします.
- mRNA配列の理解は,遺伝子発現と調節の研究に役立ちます.
- グロービンmRNAの比較分析は,進化の関係についての洞察を提供します.
研究 の 目的:
- ウサギとヒトのアルファグロービンmRNAの3'ノンコーディング領域をシーケンス化し,比較する.
- 2つの種間のシーケンスの類似点と違いを特定するために.
- 観察された配列変数の潜在的機能的意義を調査する.
主な方法:
- リバーストランスクリプターゼと (dT10) dGdCdCプライマーを用いた補完的なDNA合成.
- Sanger and Coulson plus and minus ゲル手順を用いたDNAシーケンス決定です.
- 取得したmRNAデータの比較シーケンス分析.
主要な成果:
- ウサギのアルファグロービンmRNAの完全な3'非コーディング領域 (89核酸) と部分的なコーディング領域をシーケンスした.
- 人間とウサギのアルファグロービンmRNA 3'非コーディング領域は,約80%のホモロジーを示しています.
- 人間のアルファ・グロービンmRNA配列に24ヌクレオチドの挿入が特定され,ウサギには存在しなかった.
結論:
- この高いホモロジーは,種間のアルファ-グロービンmRNAの機能が保存されていることを示唆しています.
- 人間のアルファグロビンmRNAに特定された挿入は,機能的な意味を持つ可能性があります.
- この発見は,グロービン遺伝子の進化と調節の理解に寄与する.
関連する概念動画
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
02:36Transfer RNA Synthesis
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...