まとめ
イーストのSUC2遺伝子は,RNAレベルでのグルコースレベルによって調節される2つのインバーターゼ形式を生成します. SUC2遺伝子の異なるRNAは,分泌された対細胞内逆転酶生成と相関しています.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- 酵素学 酵素学とは
背景:
- Saccharomyces cerevisiaeのSUC2遺伝子は,2つの異なるインバーターゼ酵素をコードする:分泌された,グルコース抑制によって調節されるグリコシル化形態と,構成的に生成される細胞内,グリコシル化ではない形態.
- この2つの酵素形態は,酵母による炭素代謝,特に砂糖の利用において極めて重要です.
研究 の 目的:
- SUC2遺伝子によってコードされる2種類のインバーターゼの合成と調節の差異を伴う分子機構を調査する.
- 逆転酵素合成のグルコース調節がRNAレベルで発生するかどうかを判断する.
主な方法:
- SUC2遺伝子のクローニング.
- 異なるRNA種を検出し,定量化するために,北方斑分析を行います.
- 遺伝子発現の分析 野生型および変異性酵母菌株 (例えば,SNF1ロカス変異性) の遺伝子発現の分析 異なるグルコース条件下.
主要な成果:
- SUC2遺伝子は5'端で異なる2つの異なるRNA (1.8kbと1.9kb) を生成する.
- 1.9kbのRNAは分泌されたインヴェルターゼ生成と相関し,グルコースによって調節されるが,1.8kbのRNAは細胞内インヴェルターゼと相関し,構成的に生成される.
- SNF1ロカスにおける突然変異は,分泌されたインバターゼの減圧と1.9kBのRNAの生成を阻害する.
- 静かなSUC2アレルは,正常なグルコース調節下で両方のRNAを生成する.
結論:
- イーストにおけるインヴェルターゼ合成のグルコース調節は,少なくとも部分的には,RNAレベルで媒介されます.
- 2つのSUC2RNAは,異なった転写開始部位から発生し,分泌されたまたは細胞内逆転酶の合成を決定する.
- 微分 mRNA 始動を提案するモデルは,両方のインヴェルターゼ形態の合成と調節を説明します.
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