関連する実験動画
Updated: Jul 29, 2026
09:37
Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
Published on: August 31, 2009
まとめ
ニューロブラストーマ細胞は,プラズミノゲンアクティベーターを成長コン,特にニューライトの先端で放出します. この酵素の活動は,神経細胞の移動と神経細胞の成長に不可欠である可能性があります in vivo.
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- 神経芽細胞腫は未熟な神経細胞から発生する小児がんです.
- ニューロブラストーマ細胞の移動や成長などの行動の分子メカニズムを理解することは,非常に重要です.
研究 の 目的:
- 分化ニューロブラストーマ細胞におけるプラズミノゲン活性化剤放出の特定の位置を決定する.
- ニューロンの増殖とニューロン移動におけるプラズミノゲン活性化剤の潜在的な役割を調査する.
主な方法:
- プラズミノゲン活性化剤の活性性を検出するために,フィブリンオーバーレイアッセイを用いた.
- 分化ニューロブラストーマのクローン細胞系を調べた.
主要な成果:
- プラズミノゲンアクティベーターの放出は,ニューライトを産生する細胞の72%で成長で観察されました.
- これらの細胞の21%では,成長が酵素活性の主要または唯一の部位でした.
- セレクティブ・プロテアゼの放出は,神経細胞の先端で観察された.
結論:
- 成長は,分化ニューロブラストーマ細胞におけるプラズミノゲン活性化剤の放出のための重要な部位である.
- ニューライトの先端で局所化されたプロテアースの放出は,ニューロン移動とニューライトの拡張を in vivo で促進する可能性があります.
さらに関連する動画
08:38Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Published on: May 25, 2011
13:47TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
Published on: January 29, 2015
関連する概念動画
02:47The Synapse
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information.
01:29Excitatory and Inhibitory Effects of Neurotransmitters
When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of specific...
01:19Ligand-gated Ion Channels
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
01:26Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
01:26Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...