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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

8.3K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

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タンデム質量スペクトロメトリー

F W McLafferty

    Science (New York, N.Y.)
    |October 16, 1981
    PubMed
    まとめ
    この要約は機械生成です。

    タンデム質量スペクトロメトリーは,複雑な混合物中の有機化合物を分析するための敏感で特定の方法を提供します. この技術は,高精度でピコグラムレベルの検出を迅速に達成し,他の分析方法よりも性能が優れています.

    さらに関連する動画

    Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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    Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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    Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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    科学分野:

    • アナリティカル・ケミストリー (Analytical Chemistry) とは
    • 有機化学 オーガニック・ケミストリー
    • スペクトロメトリースペクトロメトリー

    背景:

    • 複雑な混合物における特定の有機化合物の分析は,重大な課題を提示する.
    • 既存の方法は,必要な感度,特異性,または速度を欠いている可能性があります.

    研究 の 目的:

    • 特定の有機化合物を分析するための新しい技術を導入し,評価する.
    • マススペクトロメーターを連続的に結合することの利点を示します.

    主な方法:

    • タンデム質量スペクトロメトリー (MS/MS) を利用して,質量スペクトロメーターを連続的に結合する.
    • 標的化合物の選択的イオン化に続いて,最初の質量スペクトロメーターで特徴的なイオンを分離する.
    • 選択されたイオンの衝突誘発分解と,第2の質量スペクトロメーターにおける産物イオンの分析.

    主要な成果:

    • 標的化合物に対するピコグラムレベルの感受性を達成した.
    • 高い特異性とほぼ即時の反応が実証されています.
    • タンデム質量スペクトロメトリーは,放射性免疫測定法とGC/MS.に対する比較可能な特異性と感度を示した.

    結論:

    • カップル質量スペクトロメトリは,複雑なマトリックスにおける特定の有機化合物を分析するための強力な技術です.
    • この方法は,速度,感度,特異性という点で大きな利点があります.
    • タンデム質量スペクトロメトリは,確立された分析技術に有効な代替手段を提供します.