海 (S. purpuratus) のDNA配列H2A,H2B,H3ヒストンのコーディングとスペーサー領域
Cell
|November 1, 1978
まとめ
発達初期に活性化している海刺ヒストンの遺伝子が配列化されました. H2Aにメチオニンの存在は,これらのクローンDNAセグメントが胚の遺伝子発現に不可欠であることを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
背景:
- ヒストンは,ユカリオットのDNA包装に不可欠です.
- 海ヒストンの遺伝子は,繰り返し単位で編成されています.
- 特定のヒストンの変種は,初期の胚形成の間に発現します.
研究 の 目的:
- クローンされたヒストン遺伝子のDNA配列を決定するために,海S. purpuratus.からの繰り返しユニットをクローンします.
- これらのヒストン遺伝子のコーディングとスペーサー領域を分析する.
- クローンされたヒストン遺伝子の発達活動を推論する.
主な方法:
- クローンされた海ヒストンの遺伝子セグメントのDNAシーケンシング.
- アミノ酸組成のコーディング領域の分析.
- 既知のヒストンタンパク質配列とのDNA配列の比較.
主要な成果:
- H2B,H3,H2Aの遺伝子を含む2つのクローンセグメントを配列化し,横にスペースを設けました.
- H2Aタンパク質のメチオニン残留を特定し,早期発達の活性を示した.
- 偏ったコドン選択とタンパク質配列とのコリニアリティを観察し,コーディング領域の挿入がない.
- 散らばったGCクラスターとAT豊富なスペーサー領域と,規制領域でホモログな配列を保存した.
結論:
- クローンされたDNA配列は,初期の海の胚形成中に活性化しているヒストン遺伝子を表しています.
- スパイサー領域に保存された配列は,遺伝子転写またはmRNA翻訳に役割を果たす可能性があります.
- ヒストン遺伝子の重複ユニットは,H2A-H1スペーサーの特定の重複を含むユニークな構造特性を含んでいます.
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