人間のAd12とAd5の2.2kBのE1bmRNAは,異なるAUGトリプリットから始まる2つの腫瘍抗原をコードする
Cell
|November 1, 1981
まとめ
アデノウイルスE1bの初期領域は,同一の構造と重要な核酸ホモロジーを共有しています. 2つの重複する読み取りフレームは,主要な腫瘍抗原をコードし,異なるイニシアーションコドンがタンパク質のサイズを決定します.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 遺伝学 遺伝学とは
背景:
- アデノウイルス (Ads) は複合的なゲノムを持つ二重鎖DNAウイルスです.
- 初期領域E1bは,ウイルス腫瘍形成と宿主細胞の操作に不可欠です.
- アドゲノム組織を理解することは,ウイルスの複製と病原性を解読する鍵です.
研究 の 目的:
- アデノウイルス12 (Ad12) の初期領域E1bの構造的組織を決定する.
- アデノウイルス5 (Ad5) のE1b領域のヌクレオチド配列を修正し,比較する.
- E1b領域内の主要な腫瘍抗原のコーディング戦略を解明する.
主な方法:
- Ad12とAd5のE1b領域のヌクレオチド配列解析. Ad12とAd5のE1b領域のヌクレオチド配列分析. Ad12とAd5のE1b領域のヌクレオチド配列分析. Ad12とAd5のE1b領域のヌクレオチド配列分析. Ad12とAd5のE1b領域のヌクレオチド配列分析. Ad12とAd5のE1b領域のヌクレオチド配列分析. Ad12とAd5のE1b領域のヌクレオチド配列分析.
- RNA構造を定義するためのS1核酸マッピング.
- タンパク質製品を確認するためのペプチドマッピング.
- メッセンジャーRNA (mRNA) のトランスクリプトの分析.
主要な成果:
- Ad12とAd5 E1b地域の構造は同じである.
- Ad12 と Ad5 E1b.の間で相当な核酸ホモロジーがある.
- 2つの重複する読み取りフレームは,主要な腫瘍抗原 (19/21 kDaと54/55 kDa) をコードする.
- 単一の2.2kbmRNAは,両方の抗原をコードし,異なるAUGコドンで始動する.
- 1kbのmRNAは,より小さな腫瘍抗原をコードする.
- タンパク質IXのAd12アナログの特性.
結論:
- Ad12とAd5のE1b領域は,構造と配列において高度に保存されています.
- 読み取りフレームの重複による差異的翻訳開始は,異なる腫瘍抗原を生成します.
- この発見は,Ad E1bの遺伝子発現と抗原生成の詳細な分子理解を提供します.
関連する概念動画
The Central Dogma
116.7K
Overview
116.7K
Non-LTR Retrotransposons
12.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.4K
Transfer RNA Synthesis
10.2K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
10.2K
tRNA Activation
18.0K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
18.0K
Initiation of Translation
24.7K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
24.7K
Leaky Scanning
4.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
4.5K


