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Bacterial Phylum Spirochaetes
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Fv2rr骨髄からの洗浄可能なマクロモレクルは,BFU-Eのエリトロポエティック原始細胞のDNA合成を否定的に調節する
Cell
|October 1, 1981
まとめ
ネズミの遺伝因子は,初期の赤血球前駆体 (BFU-E) の負の成長調節器を調節することによって,赤血球形成を制御する. この調節体は,BFU-EにおけるDNA合成を減少させ,赤血球の発達に影響を与えます.
科学分野:
- 血液学 ヘマトロジ
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- マウスのFv2ロクスは,フレンド型赤血球白血病ウイルスに対する感受性に影響します.
- エリトロポエーシスには,BFU-E (爆裂形成ユニット-エリトロイド) のような原始細胞が含まれています.
- 早期の赤血球原始体におけるDNA合成の調節は完全に理解されていません.
研究 の 目的:
- BFU-E.におけるDNA合成の遺伝子制御を調査する.
- 初期のエリトロポエチス原始細胞の増殖を調節するメカニズムを特定する.
- Fv2局所がBFU-Eに及ぼす影響の性質を特徴づけるために.
主な方法:
- 3H-チミジンとヒドロキシ尿素を用いたインビボおよびインビトロ細胞自殺実験.
- DNA合成の変化を評価するために骨髄細胞を洗浄する.
- 抑制活性に対する細胞フリースーパーナタンスの分析.
- 抑制活性 (熱安定性,濾過,ゲル濾過) の生化学的特徴.
主要な成果:
- 耐性Fv2rrマウスのBFU-Eは,敏感Fv2ssマウスと比較してDNA合成が低いことを示しています.
- Fv2rr骨髄細胞を洗浄すると,BFU-E DNA合成がFv2ssレベルまで増加しました.
- 非毒性のある,マクロ分子負の調節剤は,Fv2rr骨髄上位体で特定され,BFU-E DNA合成を減少させました.
結論:
- Fv2の位置は,マクロ分子負の調節器を制御することによって,エリトロポエーゼスを間接的に調節する.
- この調節体はBFU-Eに作用し,DNA合成を減少させ,生理学的マイナスの成長制御メカニズムを示します.
- 初期のエリトロポエチス原始細胞の増殖のためのこのメカニズムは,厳格な遺伝子制御下にあります.
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