D. discoideumの結合に関与するグリコプロテインは,細胞表面に非ランダムな方法で分布し,細胞の結合を好む
Cell
|September 1, 1980
まとめ
この研究では,グリコタンパク質gp150がDictyostelium discoideumの集積過程で細胞との接触部位に濃縮されていることが明らかになりました. この細胞表面タンパク質は,プラズマ膜と密接に関連し,細胞と細胞の相互作用を助けます.
科学分野:
- 細胞生物学 細胞生物学
- 発達生物学 発達生物学について
- バイオケミストリー バイオケミストリー
背景:
- 細胞-細胞結合は,多細胞生物の発達に不可欠である.
- グリコプロテインは,細胞表面の相互作用と集積プロセスにおいて重要な役割を果たします.
- ディクティオステリウム・ディスコイデウム (Dictyostelium discoideum) は,細胞の集積を研究するためのモデル生物として機能しています.
研究 の 目的:
- Dictyostelium discoideumにおけるグリコプロテインgp150の細胞表面分布を調査する.
- 細胞集積過程におけるgp150の役割を理解する.
主な方法:
- 健全なディクチオステリウム・ディスコイデウム集積物の免疫ホモシアニンラベル付け.
- スキャン電子顕微鏡 (SEM) を使用した可視化.
- 詳細な表面分析のための免疫フェリチンラベリングと伝送電子顕微鏡 (TEM).
主要な成果:
- 細胞表面でgp150の明確な分布グラデーションが観察されました.
- gp150濃度は,細胞と細胞の接触地点またはその近くで有意に濃縮された.
- gp150は,プラズマ膜と密接に関連していることが判明しました.
結論:
- gp150は細胞と細胞の接触領域に局限しており,Dictyostelium discoideumの集積に役割を果たしていることを示唆しています.
- gp150のプラズマ膜との結合は,細胞結合におけるgp150の機能を促進する.
関連する概念動画
Mechanism of Filopodia Formation
2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
Matrix Proteoglycans and Glycoproteins
4.3K
Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
4.3K
Adherens Junctions
5.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
5.8K
Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
Glycocalyx and its Functions
9.2K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
9.2K
Diversity of Protists IV
2.1K
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
2.1K


