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Updated: Jul 16, 2026

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

アデノウイルスメジャー・ラット・トランスクリプション・ユニットからのトランスクリプトの早期キャッピング

A Babich, J R Nevins, J E Darnell

    Nature
    |September 18, 1980
    PubMed
    まとめ

    RNAキャピングは,アデノウイルス2型 (Ad-2) RNA処理の初期のイベントであり,転写開始直後に急速に発生します. これは,RNAキャピングがすべてのRNAポリメラーゼII産物の自動プロセスであり,mRNA成熟の選択的なステップではないことを示唆しています.

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    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...