関連する実験動画
Updated: Jul 29, 2026

07:51
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Published on: May 21, 2015
まとめ
ネズミの妊娠中の母親によるアロ抗体の生成は,H-2ハプロタイプと関連しています. 主要な抗体はIgG1であり,細胞毒性のない抗体であり,潜在的に保護効果を発揮する可能性がある.
科学分野:
- 免疫学 免疫学とは
- 生殖生物学 生殖生物学
- 遺伝学 遺伝学とは
背景:
- 胎児の抗原に対する母体免疫反応は哺乳類でも発生しますが,その意義は不明です.
- ネズミでは,父親から受け継がれる胎児抗原に対する抗体の生成は,株とH-2ハプロタイプに依存しています.
- 以前の研究では,母性アロ抗体の細胞毒性活性が示唆されており,この研究では再評価されています.
研究 の 目的:
- ネズミの妊娠中に産生される母性アロ抗体の特定の免疫グロブリン (Ig) Gサブクラスを特定するために.
- これらのアロアンチボディの機能的特性,特に細胞毒性を決定する.
- 妊娠中の母親のアロ抗体反応の生物学的な関連性を解明する.
主な方法:
- ヘマグルーチネーションアッセイは,胎児ヒストコンパティビリティ抗原に対するアロ抗体を検出する.
- プラズマサイトマから派生した同型特異のアンチセラを用いた改変した血液吸収測定法.
- 母親の血清における補足依存性サイト毒性の評価.
主要な成果:
- 母親のアロ抗体の産生は,近親性のマウス株における特定のH-2ハプロタイプと関連しています.
- 母親の血清では,補足に依存した有意な細胞毒性活動は検出されなかった.
- 主要なアロ抗体反応は,非補足固定性であるIgG1サブクラスを含む.
- ネズミの妊娠は,細胞毒性のないIgG1アロ抗体を誘発する.
結論:
- ネズミの妊娠における主要な母性アロ抗体の反応は,細胞毒性のないIgG1サブクラスによって媒介されます.
- このIgG1アロアンチボディは,有害ではなく,免疫強化または保護的特性を有している可能性があります.
- この発見は,母子免疫界面と特定の抗体サブクラスの役割の理解に貢献します.
さらに関連する動画
07:13Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C)
Published on: March 25, 2016
09:09Generating a Reproducible Model of Mid-Gestational Maternal Immune Activation using Poly(I:C) to Study Susceptibility and Resilience in Offspring
Published on: August 17, 2022
関連する概念動画
Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Transcytosis of IgG
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Antibody Structure and Classes
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
Antibody Actions
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Development of Immunocompetence
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...