神経および心臓の発達における神経ルリン受容体 erbB2 の必要性

K F Lee¹, H Simon, H Chen

¹Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037, USA.

Nature

|November 23, 1995

まとめ

表皮成長因子受容体2 (erbB2/neu) は哺乳類の発達に不可欠である. マウスにおける erbB2 機能の喪失は,胚の死亡と心臓および神経の発達に重大な欠陥をもたらします.

科学分野:

発達生物学発達生物学とは
分子生物学は分子生物学である.
腫瘍学腫瘍学

背景:

皮膚増殖因子受容体2 (erbB2/neu) は癌に関与しており,遺伝子の増幅は乳がんや卵巣がんの予後不良に関連している.
ニューレグルインは,erbB受容体と相互作用するリガンドであり, erbB2はシグナル伝達のために他のerbBファミリーメンバーとの異体化を必要とすると考えられています.

研究の目的:

哺乳類の胚発達におけるerbB2の重要な役割を調査する.
erbB2.2の欠如によって影響を受ける特定の発達過程を明らかにする.

主な方法:

erbB2のヌルアレルを持つマウスの生成と分析.
胚の死亡率,心臓の発達 (トラベキュラ形成),神経の発達 (感受性カンジリア,運動神経) の検査.
運動神経の発達を評価するために,DIIの逆行トレースを使用した.

主要な成果:

機能的なerbB2 (erbB2ゼロアレル) を欠いたマウスは,胚の11日前までに胚死亡率を示した.
心臓の発達に重大な欠陥が認められ,特にトラベキュラが欠けていた.
頭蓋のニューラル・クライストから派生した感覚性ガングリアの発達が著しく低下した.
運動神経の発達も,erbB2変異体胚では損なわれていました.

結論:

この研究は,哺乳類の胚形成過程における心臓と神経の両方の発達において,erbB2の重要な重要性を示しています.
これらの発見は, erbB2 が癌における既知の役割を超えて,重要な発達経路の重要な調節因子であることを強調しています.

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