Bcl-2は抗酸化経路で機能し,アポプトシスを防ぐ
D M Hockenbery1, Z N Oltvai, X M Yin
1Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110.
Cell
|October 22, 1993
まとめ
抗アポプトシスタンパク質Bcl-2は,抗酸化経路を調節することによって,プログラム細胞死を抑制する. 細胞を酸化的ダメージ,特に脂質過酸化から,フリーラジカルの発生部位で保護する.
科学分野:
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
背景:
- アポトーシス,またはプログラム細胞死は,基本的な生物学的プロセスです.
- Bcl-2タンパク質ファミリーは,アポトーシスを調節する上で重要な役割を果たします.
- Bcl-2は細胞死を抑制することが知られているが,その正確なメカニズムはまだ調査中である.
研究 の 目的:
- 酸化性細胞死を予防するBcl-2の役割を調査する.
- Bcl-2が抗アポプトティック効果を発揮するメカニズムを解明する.
- Bcl-2が細胞内の抗酸化経路を調節するかどうかを判断する.
主な方法:
- アポトーシスの細胞モデルを用いて.
- 酸化ストレス (H2O2,メナディオン) によって誘発される細胞死に対するBcl-2過剰発現の影響を評価.
- 反応性酸素種生成と脂質過酸化レベルを測定する.
主要な成果:
- Bcl-2はアポプトティック細胞死,特に過酸化水素 (H2O2) とメナディオンによって引き起こされる死を抑制しました.
- N-アセチルシステインやグルタチオンペロキシダゼなどの過酸化物を除去する抗酸化物質は,アポプトシスによる死に対抗したが,マンガン超酸化物ディスミュータゼはそうしなかった.
- Bcl-2過剰発現は,アポプトティック細胞死の一つの特徴である脂質過酸化を抑制し,細胞を特定の細胞内部位での酸化的損傷から保護しました.
結論:
- Bcl-2は,細胞内抗酸化経路の調節体として機能する.
- Bcl-2の抗アポプトシス作用は,脂質過酸化を抑制する能力と関連しています.
- Bcl-2は,フリーラジカルの生成を調節し,細胞の重要な部位でスキャビングすることで,保護効果を発揮する可能性がある.
関連する概念動画
01:24Other Glycolytic Pathways
914
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
914
01:30Apoptosis
15.1K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.1K
01:27C4 Pathway and CAM
49.2K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
49.2K
02:59Cancer Prevention
8.1K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
8.1K
01:14Prevention of Further Absorption of Poison
1.3K
In cases of acute poisoning, the primary objective is to prevent further absorption of the toxic substance into the body. Immediate interventions using various decontamination techniques targeting the gastrointestinal (GI) tract can achieve this. Decontamination is crucial to prevent poison from entering the systemic circulation, which involves washing affected areas with water and mild soap and removing contaminated clothing. Once external decontamination is done, attention must be turned to...
1.3K
01:30Preventive Healthcare Services
2.2K
Preventive healthcare services keep people healthy via frequent check-ups, screening, and counseling. They primarily aid in disease prevention rather than treating an acute or chronic illness. Preventive treatment also keeps individuals productive and energetic, allowing them to work well into their retirement years. Examples of preventive care services include:
2.2K