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関連する概念動画

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

7.0K
Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

7.5K
This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

18.9K
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
18.9K
Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

21.6K
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
21.6K
DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

21.0K
RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

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We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
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関連する実験動画

Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

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ユカリオット生物における誤った翻訳

E Palmer, J M Wilhelm

    Cell
    |February 1, 1978
    PubMed
    まとめ

    パロマミンを含むアミノグリコシド抗生物質は,シリアト・テトラヒメナ・サーモフィラの成長とタンパク質合成を阻害する. また,これらの薬剤は誤翻訳を誘発し,遺伝学の研究の可能性を秘めています.

    科学分野:

    • 微生物学 微生物学とは
    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは

    背景:

    • アミノグリコシド抗生物質,特にパロマミン断片を持つ抗生物質は,タンパク質合成の誤りを誘発することが知られている.
    • ユカリオット細胞のないシステムは,以前にこれらの誤翻訳抗生物質に対する感受性を示しています.

    研究 の 目的:

    • パロマミンを含むアミノグリコシド抗生物質に対するシリアト・テトラヒメナ・サーモフィラの感受性を調査する.
    • これらの抗生物質の成長,タンパク質合成,およびテトラヒメナにおける翻訳的忠実性への影響を評価する.

    主な方法:

    • Tetrahymena thermophilaの培養物にパロマミンを含むアミノグリコシドを投与する.
    • 成長抑制とタンパク質合成の速度を測定する.全生物と細胞のない抽出物.
    • 細胞フリータンパク質合成システムを用いて誤翻訳誘導の評価.

    主要な成果:

    • Tetrahymena thermophilaは,パロマミンを含むアミノグリコシドに対して敏感性を示しています.
    • 抗生物質は,生物の成長とタンパク質合成の両方を in vitro と in vivo で抑制します.
    • Tetrahymenaから派生した細胞フリー系において,誤翻訳の有意な刺激が観察されました.

    さらに関連する動画

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

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    A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
    11:53

    A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

    Published on: July 12, 2017

    18.9K

    関連する実験動画

    Last Updated: Jan 14, 2026

    In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
    07:52

    In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

    Published on: November 2, 2020

    7.0K
    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    7.5K
    A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
    11:53

    A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

    Published on: July 12, 2017

    18.9K

    結論:

    • パロマミンを含むアミノグリコシドは,テトラヒメナ・サーモフィラに対して効果があり,成長とタンパク質合成に影響を与えます.
    • これらの抗生物質の誤翻訳特性から,翻訳的に抑制された変異を持つ菌株を隔離する際の有用性が示唆される.
    • この研究は,これらの薬剤が遺伝子研究,特に誤った意味と無意味な変異を研究する上で持つ可能性を強調しています.