人間のBリンパ球によって生成される酸化窒素は,アポトーシスとエプスタイン・バーウイルスの再活性化を阻害する
J B Mannick1, K Asano, K Izumi
1Department of Microbiology and Molecular Genetics, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Cell
|December 30, 1994
まとめ
酸化窒素 (NO) は,ヒトB細胞で構成的に発現し,アポトーシスを抑制し,エプスタイン・バーウイルス (EBV) の潜伏期を維持する上で重要な役割を果たします. この発見は,NOのNOを明確にします.
科学分野:
- 免疫学 免疫学とは
- ウイルス学 ウイルス学 ウイルス学
- 細胞生物学 細胞生物学
背景:
- 酸化窒素 (NO) は,ウイルス感染症に対するマウリンの耐性にとって極めて重要です.
- 人間の免疫反応,特に単核細胞におけるNOの役割は不明である.
- エプスタイン・バーウイルス (EBV) はヒトBリンパ球に感染し,潜伏期を確立します.
研究 の 目的:
- ヒトBリンパ球における酸化窒素合成酵素 (NOS) の発現と機能を調査する.
- EBVの潜伏期およびB細胞アポトーシスにおけるNOの役割を決定する.
主な方法:
- EBV変異Bリンパ球とバーキットリンパ腫細胞系における誘導性酸化窒素合成酵素 (iNOS) 発現の評価.
- NOがEBVのトランザクティベーターZta表現に与える影響を調査した.
- NOがアポトーシスとcGMP独立のシグナル伝達経路に及ぼす影響を調べました.
主要な成果:
- 構成的,低レベルのiNOS発現は,ヒトBリンパ球細胞系で検出されました.
- NOの生成はZta発現をダウンレギュレーションし,EBVの潜伏期を維持することが判明しました.
- NOは,cGMPから独立して,Bリンパ球細胞系におけるアポトーシスを抑制した.
結論:
- NOは,ヒトのB細胞生物学において生理学的役割を果たします.
- NOは,B細胞におけるプログラム細胞死の抑制に寄与する.
- NOは,ヒトB細胞のウイルス潜伏期,特にEBVの維持に関与しています.
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