RIP:死領域を含む新しいタンパク質で,酵母におけるFas/APO-1 (CD95) と相互作用し,細胞死を引き起こす
B Z Stanger1, P Leder, T H Lee
1Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Cell
|May 19, 1995
まとめ
研究者は,Fas受容体と相互作用し,プログラム細胞死 (アポトーシス) を誘導する新しいタンパク質,受容体相互作用タンパク質 (RIP) を特定しました. この発見は,細胞死シグナル伝達経路の分子メカニズムに光を当てています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 免疫学 免疫学とは
背景:
- ファス/APO-1 (CD95) 受容体結合はプログラム細胞死を誘発する.
- Fasの細胞内シグナル伝達経路を理解することは,細胞死の研究にとって極めて重要です.
研究 の 目的:
- Fas受容体の細胞内領域と相互作用するタンパク質を特定する.
- 新しいFas相互作用タンパク質の機能と特性を特徴付ける.
主な方法:
- タンパク質とタンパク質の相互作用をスクリーニングするための酵母2ハイブリッドシステム.
- 相互作用を確認するために共免疫プレシピテーションとウエスタン・ブロッティング.
- アポトーシスの誘導を評価するために,一時的な転移と形態学的分析.
主要な成果:
- 新しい74 kDaのタンパク質,受容体相互作用タンパク質 (RIP) を特定し,Fas細胞内領域に結合した.
- RIPはまた,腫瘍死滅因子受容体1 (TNFR1) と相互作用し,死亡ドメインを含んでいます.
- RIPの過剰発現は,感染した細胞でアポトーシスを誘発する.
結論:
- RIPは,Fas誘発のアポトーシスを媒介する新しいタンパク質です.
- RIPは,プログラムされた細胞死経路において重要な役割を果たします.
- RIPは,アポトーシスを誘発するタンパク質の新しいクラスを表しています.
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