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転写因子EBFが欠けているマウスにおけるB細胞の微分化の失敗
1Howard Hughes Medical Institute, Department of Microbiology and Immunology, University of California, San Francisco 94143, USA.
Nature
|July 20, 1995
まとめ
初期のB細胞因子 (EBF) は,Bリンパ球の発達に不可欠です. EBF欠乏したマウスは,免疫グロブリン発現するB細胞を欠いており,B細胞の分化におけるEBFの重要な役割を強調しています.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
背景:
- 早期B細胞因子 (EBF) は,Bリンパ球の分化に不可欠な転写因子である.
- EBFは,B細胞発達の過程でmb-1のような遺伝子を調節する.
研究 の 目的:
- 免疫グロブリン発現B細胞の生成のためにEBFの必要性を調査する.
- B-リンパ球の分化におけるEBFの特定の役割を決定する.
主な方法:
- EBF欠乏モデルを作成するためにマウスの標的型遺伝子破壊.
- 変異したマウスのB細胞集団と遺伝子発現の分析.
主要な成果:
- EBF欠乏マウスは,免疫グロブリンDとJHの遺伝子セグメントを再配置したB細胞を欠いている.
- ミュータントマウスの原始細胞 (B220+CD43+) は,生殖細胞のmuとIL-7受容体のトランスクリプトを発現する.
- 嗅覚ニューロンを含むEBFを発現する非リンパ性組織は影響を受けません.
結論:
- EBFは,免疫グロブリン発現するB細胞の生成に不可欠です.
- EBFは,B細胞の微分化の転写制御において重要な役割を果たします.
- EBFの機能は,B細胞の発達に特異的であり,系統のコミットメント後に発生するが,Ig遺伝子の再編成の前に発生する.
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