IgE依存ヒスタミン放出因子の分子識別
S M MacDonald1, T Rafnar, J Langdon
1Department of Medicine, Johns Hopkins University School of Medicine, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.
Science (New York, N.Y.)
|August 4, 1995
まとめ
研究者らは,アレルギー患者の免疫グロブリンE (IgE) と関連したヒスタミン放出因子 (HRF) を特定した. この因子であるHRFは慢性アレルギー疾患に作用し,ベースフィルからヒスタミンの放出を引き起こします.
科学分野:
- 免疫学 免疫学とは
- アレルギー研究 アレルギー研究
背景:
- アレルギー疾患には,複雑な免疫反応が伴う.
- 免疫グロブリンE (IgE) は,アレルギー反応において中心的な役割を果たします.
- ヒスタミンの放出は,アレルギー反応の重要な媒介です.
研究 の 目的:
- IgE依存性アレルギー反応に関与するヒスタミン放出因子 (HRF) を特定し,浄化する.
- HRF,IgE,ヒスタミン放出との関係を調査する.
- 慢性アレルギー疾患におけるHRFの役割を調査する.
主な方法:
- アレルギー患者の生体液からHRFを浄化する.
- HRFのアミノ端末配列.
- 再結合HRFの生産.
- ヒトベースフィルのヒスタミン放出活性に対する再結合HRFのテスト.
- ポリクローン抗体を用いてHRFの活性を中和する.
主要な成果:
- HRFを特定し,アトピー性子供のリンパ球とアレルギー患者の生体液から浄化しました.
- HRFはマウスp21とヒトp23.3との同質性を示した.
- リコンビナントHRFは,ヒトベースフィルのサブ集団からIgE依存ヒスタミン放出を誘発した.
- 抗体はHRFの生物学的活動を効果的に認識し,除去しました.
結論:
- HRFはヒスタミン放出のIgE依存のメディエーターである.
- HRFはIgEの異質性に関連しています.
- HRFは慢性アレルギー疾患の病原化に関与しています.
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