HIV-1 Revの活性化ドメインは,特定の細胞RNAによって使用される輸出経路にアクセスする核輸出信号です

U Fischer¹, J Huber, W C Boelens

¹Institut für Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany.

Cell

|August 11, 1995

まとめ

HIV-1 Revタンパク質であるHIV-1 Revは,

科学分野:

分子生物学は分子生物学である.
ウイルス学ウイルス学ウイルス学
細胞生物学細胞生物学

背景:

ヒト免疫不全ウイルス1型 (HIV-1) のRevタンパク質は,ウイルスの複製に不可欠です.
Revは,未結合および部分結合のウイルスRNAの核輸出を促進し,これは新しいビリオンの生成に不可欠です.

研究の目的:

HIV-1 Revタンパク質が核RNAの輸出を媒介するメカニズムを解明する.
核の輸出を担うRevタンパク質の特定の機能領域を特定する.

主な方法:

使用された牛の血清アルブミン (BSA) の結合体とRev活性化ドメイン (BSA-R) のペプチド.
様々なRNA種とリボソームサブユニットのRev媒介核輸出に対するBSA-Rの影響を評価した.
BSA-R自体の核輸出を調査した.

主要な成果:

BSA-Rは,RREを含むRNAのRev媒介核輸出を抑制したが,変異したバージョンはそうしなかった.
BSA-Rは,5S rRNAとU snRNAのエクスポートを特に抑制したが,mRNA,tRNA,またはリボソームサブユニットのエクスポートは抑制しなかった.
BSA-Rは,原子核から活発かつ飽和的に輸出された.

結論:

HIV-1 Revの活性化ドメインは,核輸出信号 (NES) として機能します.
このNESは,RREを含むウイルスRNAを,mRNA以外の核輸出経路にリダイレクトします.
この発見は,ウイルスRNAの輸出と核輸送の分子メカニズムについての洞察を提供します.

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