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NSFのようなATPase,p97とNSFは,ミトのゴルギ断片から水槽の再生を媒介する
C Rabouille1, T P Levine, J M Peters
1Cell Biology Laboratory, Imperial Cancer Research Fund, London, England.
Cell
|September 22, 1995
まとめ
Golgi cisternaeは,特定のタンパク質を使用してミトーシス後に再生することができます. NSF,SNAP,p115は,ATPase p97と共に,ゴルギの構造と機能を再構築するために不可欠です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- タンパク質生化学 タンパク質生化学
背景:
- ゴルギ装置はミトーシスで分解され,子細胞のために再構築されなければならない.
- ゴルギの構造を回復する分子機構を理解することは,細胞分裂に不可欠です.
研究 の 目的:
- ミトスのゴルギ断片の細胞フリー再生に関与する重要なタンパク質を調査する.
- ゴルジ改革におけるp97とNSF-SNAPs-p115複合体の異なる役割を明らかにする.
主な方法:
- ミトのゴルギの断片を持つ細胞のないシステムを利用した.
- 適用されたN-エチルマレイミド (NEM) または塩洗浄は,再生を抑制します.
- 浄化されたp97 (ATPアゼ) またはNSF (N-エチルマレアミド感受因子) とSNAP (溶性NSF付着タンパク質) とp115 (膀ドッキングタンパク質) を使って再生を回復した.
主要な成果:
- ゴルギの水槽は,細胞のないシステムで自発的に再生しました.
- NEMや塩洗いによる再生抑制は,p97またはNSF-SNAPs-p115複合体を加えることで克服されました.
- NSF-SNAPs-p115と比較して,p97で再生された水槽では,異なる形態が観察され,差異的な機能を示した.
結論:
- p97とNSF-SNAPs-p115複合体は,ミトーシス後のゴルギのリフォームに不可欠である.
- これらのタンパク質複合体は,ゴルジ水槽の構造再構築において,異なる非冗長な役割を果たします.
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