アラビドプシスの花の形成を誘発する遺伝子
1Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
Nature
|October 12, 1995
まとめ
アラビドプシスのAPETALA1 (AP1) 遺伝子を構成的に発現させることで,芽を花に変えます. この初期の開花反応は,AP1が示しています.
科学分野:
- 植物発達生物学 植物発達生物学
- 分子遺伝学 分子遺伝学
- アラビドプシスの研究
背景:
- アラビドプシスのアピカルシューツメリスタムは,シューツまたは花に微分化する横向メリスタムを生成します.
- APETALA1 (AP1) や LEAFY (LFY) を含む花のメリシステムアイデンティティ遺伝子は,花の運命を決定する上で極めて重要です.
研究 の 目的:
- メリシステム運命を決定するAPETALA1 (AP1) の役割を調査する.
- AP1単独で花のメリステムのアイデンティティを誘発し,花開の時間に影響を与えるかどうかを判断する.
主な方法:
- AP1遺伝子の構成的発現を持つトランスジェニックアラビドプシス植物を生成する.
- トランスジェニック植物における芽とメリステムの変容の観察と分析.
- トランスジェニック植物と野生型の植物の開花時間を比較する.
主要な成果:
- AP1を構成的に発現するトランスジェニック植物は,アピカル・シューツとサイド・シューツの両方の変異を花に表した.
- これらのAP1過剰発現する植物は,野生型のアラビドプシス植物と比較して,著しく早い開花を示した.
- エクトピックAP1発現は,花束シューツメリスタムを花のメリスタムに変換するのに十分でした.
結論:
- APETALA1 (AP1) は,アラビドプシスの花のメリスタムのアイデンティティの重要な決定因子である.
- AP1の構成的発現は,正常な発達経路を覆し,シューツからフラワーへの変換につながる可能性があります.
- AP1は,開花期への移行を調節する上で重要な役割を果たします.
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