プロフェロモンの処理と芽の場所の選択における酵母インスリン分解酵素のホモログの役割
N Adames1, K Blundell, M N Ashby
1Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
Science (New York, N.Y.)
|October 20, 1995
まとめ
Saccharomyces cerevisiae AXL1遺伝子の産物であるAxl1pは,a-因子フェロモン分泌と軸芽部位の選択の両方に重要である. AXL1の変異は,これらのプロセスを破壊し,Axl1pを強調します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- イースト遺伝学 イースト遺伝学
背景:
- Saccharomyces cerevisiae AXL1の遺伝子産物であるAxl1pは,インスリン分解酵素であるエンドプロテアゼのファミリーと同質性を示しています.
- Axl1pは酵母細胞の機能に作用し,タンパク質の処理と細胞形態学を潜在的に含む.
研究 の 目的:
- Saccharomyces cerevisiaeにおけるAxl1pの二重機能を調査する.
- A-ファクターフェロモン分泌と芽部部位選択におけるAxl1pの役割を明らかにする.
- Axl1pの活性部位が機能に与える特定の貢献度を測定する.
主な方法:
- A因子フェロモン分泌の欠陥を評価するために,酵母 axl1変異体の分析.
- a因子前駆体内の加工所を特定する.
- 軸芽部部位の選択におけるAxl1pの役割の調査.
- Axl1p.の推定活性部位のサイト指向型変異.
主要な成果:
- 酵母 axl1変異体は,a-因子フェロモン分泌に重大な欠陥を示した.
- Axl1pの潜在的な処理部位は,a-ファクター前駆体で特定されました.
- Axl1pは,軸芽部部位選択の形態遺伝的決定因子として関与していた.
- Axl1p活性部位におけるアミノ酸置換はプロフェロモン処理を阻害したが,芽部位の選択を阻害しなかった.
結論:
- Axl1pは,Saccharomyces cerevisiaeの異なるシグナル伝達経路の二重調節に関与する.
- Axl1pはプロフェロモン処理と細胞の極性調節の両方に関与しています.
- この研究は,重要なプロペプチド処理イベントにインスリン酵素ファミリーのメンバーを関与させる.
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